Nordic Seed's laboratory services

Lean more about Nordic Seed's laboratory services below.
 
Click on the plus icons to open the topic for more details about germination, purity tests and production of doubled haploids.

 

For more details, please contact our laboratory staff. You will find their contact information here.

 

Laboratory

 


Laboratory services

  • + DH plant production

    The production of doubled haploid (DH) plants is a technique where immature pollen cells are isolated and grown on a nutrient medium. The result hereof will be gametes with half the number of chromosomes. Since this condition is unstable, the plant will in most cases double the number of chromosomes spontaneously and then become a doubled haploid plant. This plant is 100% homozygote and you can skip several rounds of self-pollination.

    Typically, the laboratory receives F1 seeds from breeders, which are sown in our greenhouse. Immature spikes are harvested, when they are in the right stage. The spikes are sterilised, prepared and pre-treated – often a cold treatment. The immature pollen cells are isolated by either anther or microspore culture. Part of the immature pollen cells will develop into embryos and part of them will develop into plants. When the plants reach a certain stage, they are removed into soil.  

    For more information please read the brochure with a specific review of microspore culture. For any further questions please contact the laboratory.

     

  • + Germination in paper

    Germination in paper of barley, wheat, rye, triticale and wheat, is used to examin the seeds germination.

    4 x 100 seeds are counted and placed between damp filter paper for 7 days at 20 degrees.

    The number of normal and abnormal, fresh non-germinated and dead seeds is counted and registered. 

    Read more about germination and certified seeds here

  • + TZ analysis

    TZ in germinating barley, wheat, rye and triticale

    TZ analysis is a germination test which can be performed within 24 hours and is often used when time is a factor. 

    TZ stands for Tetrazolium, which stains living cells in the kernels. The formation of roots and leaves is evaluated. Potential heat damage can be detected using this analysis.

    Read more about germination and certified seeds here

  • + Purity analysis

    This purity test is used to determine the purity of the seeds.

    120 grams are controlled for waste and 1000 grams is checked for seeds of other crops and weeds, including Avena fatua and Avena sterilis.

    Read more about germination and certified seeds here

  • + Molecular laboratory

    With confidence, molecular breeding at Nordic Seed is moving from the research and development phase into the application phase. 

    Our molecular breeding lab is equipped with state-of-the-art instruments, including a robot for liquid handling and a well-established single-nucleotide polymorphism (SNP) genotyping platform.  
     
    Our overall aim in the Research & Development team is to provide breeders with relevant molecular markers data, i.e. resistance to diseases and superior quality. Such information helps breeders to take informed decisions during selection, and to deliver high-yielding varieties with a high degree of resistance and quality that meet market demands now and in the future.
     
    Projects and collaboration with public and private partners are essential to our R&D policy. They provide important information concerning new cutting-edge technology and know-how, as well as giving Nordic Seed access to expertise and facilities from universities and other project partners..
     
    Among these national and international projects, we have industrial PhD and post-doctoral projects running at Nordic Seed to improve resistance and quality of wheat and barley.
     
    You can read more about our work here